Cell membranes can also blend or merge without fusion of the cellular contents. Penaeus monodon mature and breed only in tropical marine habitats and spend their larval, juvenile, adolescent and subadult stages in coastal estuaries, lagoons or mangrove areas. Request pdf prevalence of monodon baculovirus mbv in wild shrimp. Request pdf improvement of pcr method for the detection of monodon baculovirus mbv in penaeid shrimp penaeus monodontype baculovirus mbv. Mbv monodon baculovirus the occlusion bodies ob formed as monodon baculovirus mbv replicate are easily visible using a microscope. Monodon baculovirus has been known to be invasive in 85100% of the shrimp hatcheries, in early or late stages of shrimp. Attempts at producing a hybridised penaeus mondon cell line by cellular fusion. Penaeus monodon baculovirus mbv is a rodshaped virus possessing a. White spot baculovirus syndrome in the indian shrimp. Baculovirus infections may result in disease in cultured penaeids that is accompanied by high mortality rates. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported. Environmental assessment report import of specific.
The animal diversity web is an educational resource written largely by and for college students. The multiplex pcr master mix comprised of 2 l of 10x primer mix. Penaeus monodon, commonly known as the giant tiger prawn or asian tiger shrimp is a marine. Mbv virions are rodshaped, nonoccluded or occluded. The genome and occlusion bodies of marine penaeus monodon. Monodon baculovirus mbv disease viral infections in. Mbv virions are rodshaped, nonoccluded or occluded and enveloped within a paracrystalline occlusion body. Pdf prevalence of white spot syndrome virus and monodon. Multiple viral pathogens occurrence in tiger shrimp.
The effect of viral infection on the relationship between. Even though mbv is not a severe pathogen for the black tiger prawn penaeus monodon, it should be eliminated from the farming system because it is unlikely that. Prevalence of white spot syndrome virus wssv and monodon baculovirus mbv infection in penaeus monodon postlarvae in vietnam. There appear to be more ob when pl are stressed, so. Current status of viral diseases in asian shrimp aquaculture. The pcr reactions were done as described in oie manual of diagnosis for aquatic animals 28. The open access israeli journal of aquaculture bamidgeh. Monodon baculovirus mbv is a member of nucleopolyhedrosis virus npv group that infect penaeid shrimps. Penaeus monodon baculovirus variously known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv or monodon baculovirus mbv, the causative agent of spherical baculovirosis in shrimp p.
Improvement of pcr method for the detection of monodon. Analysis of the genome of the sexually transmitted insect. Occurrence of viral pathogens in penaeus monodon post. Pdf decontamination of monodon baculovirus in marine. Monodon baculovirus mbv exists in populations of the indian tiger prawn, penaeus monodon, obtained from a commercial hatchery in madras, india. The cytopathology, virogenesis and replication of monodon baculovirus mbv in penaeus monodon from australia are described. Necrosois virus, monodon baculovirus, white spot syndrome virus. Diseases such as whitespot baculovirus and yellowhead virus have led to a great.
Several investigators have demonstrated that prawn primary cell cultures can support in vitro growth of monodon baculovirus. However, hznv2 lacks several key genetic features of baculoviruses including the late transcriptional regulation factor, lef1. Manual of histological staining methods of the armed forces. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. Occurrence of viral pathogens in penaeus monodon postlarvae.
It is a nuclear polyhedrosis virus npv of the family. Decontamination, monodon baculovirus, mbv, shrimp egg, upwelling flowthrough system. The israeli journal of aquaculture bamidgeh 6, 2009 fig. Monodon baculovirus mbv disease viral infections in penaeid shrimps affected shrimps exhibit palebluishgray to dark blueblack coloration, sluggish and inactive swimming movements, loss of. In hatcheries, bp and bmn often cause serious epizootics in the larval and early. Adw doesnt cover all species in the world, nor does it include all the latest scientific. Decontamination of monodon baculovirus in marine shrimp. Occurrence of viral pathogens in penaeus monodon postlarvae from aquaculture hatcheries toms c. The pcr was shown to be specific for mbv dna by not amplifying prawn dna or dna preparations of baculovirus penaei bp, white spot baculovirus wsbv, bennettae baculovirus and insect. The objective of our study was to explain the histopathological changes of monodon baculovirus mbv in hepatopancreas and midgut tissues of the cultured penaeus vannamei. Penaeus monodon is the secondmost widely cultured prawn species in the world, after only whiteleg shrimp, litopenaeus vannamei.
Us6143547a cell line derived from penaeus monodon and a. Two years later, it was designated monodon baculovirus mbv 2. Another less accurate process, microhomology mediated end joining is also used to. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely.
White spot baculovirus syndrome in the indian shrimp penaeus monodon and p. Spherical baculovirosis penaeus monodon type baculovirus p. We report the use of recombinant baculovirus mediated transduction using hybrid promoter system for transgene expression in lymphoid cells of penaeus monodon. Detection of monodon baculovirus and whitespot syndrome. Colourimetric pcrbased detection of monodon baculovirus. Attempts at producing a hybridised penaeus mondon cell. Production was dominated by penaeus monodonuntil 2002 and by penaeus vannamei thereafter. Penaeus monodontype baculovirus mbv is found in many parts of the world from. Domestication of black tiger shrimp penaeus monodon in recirculation. The trees were found by a heuristic search starting with neighbor joining and nearestneighbor. A record and prevalence of monodon baculovirus from. Poor quality seed, coupled with the slow growth of p. The present study attempts to investigate the effect of penaeus monodon.
Polymerase chain reaction pcr assays have been reported for specific and accurate. It is a rodshaped, enveloped, doublestranded dna virus, and considered till recently, as the type a baculovirus. The simultaneous presence of monodon baculovirus mbv and whitespot syndrome virus wssv in apparently healthy postlarvae of penaeus monodon from different hatcheries in india was studied by. More than 600 recombinant genes have been expressed in baculoviruses to date. Shrimp, penaeus semisulcatus, penaeus monodon baculovirus, histopathology.
Decontamination of monodon baculovirus in marine shrimp eggs using upwelling flowthrough system article pdf available july 2015 with 110 reads how we measure reads. Onfarm feed management practices for black tiger shrimp. Focusing on the giant tiger shrimp penaeus monodon. Wssv, yellow head virus yhv, monodon baculovirus mbv and to a lesser extent, luminescent bacterial infections. Ultrastructural and pathogenesis of monodon baculovirus in.
Major viral diseases of penaeus monodon in taiwan chufang lo, yunshiang chang, shoen peng and guanghsiung kou received, march 10, 2003. Monodon baculovirus mbv infects epithelia1 cells of the hepatopancreas hp and midgut of penaeus monodon and other penaeid shrimp. In the wild, they show marked nocturnal activity, burrowing into bottom substratum during the day and emerging at night to search for food as benthic feeders. Monodon baculovirus mbv is an economically important and one of the. Millions of metric tons dominated by penaeus monodon. Pdf penaeus monodon type baculovirus mbv is a horizontal transmission virus disease especially found in marine shrimp.
Prevalence of monodon baculovirus mbv in wild shrimp penaeus. Pdf prevalence of white spot syndrome virus wssv and. This cell line is susceptible to and capable of mass producing virus, which includes, but not limited to, white spot syndrome virus wssv, panaeus monodon baculovirus. A survey on the presence of the viruses of two economically significant diseases, white spot syndrome virus wssv and monodon baculovirus mbv in wild. Monodon baculovirus mbv mbv is the first reported virus of p. Two years later, it was designated monodon baculovirus mbv 2, and this name is still commonly used today. Another less accurate process, microhomologymediated end joining is also used to. Guide to baculovirus expression vector systems bevs and. A recent morphological description of monodon baculovirus might correspond to this virus 47. Freepenaeus monodon hatchery manual based on the experience in india. Decontamination of monodon baculovirus in marine shrimp eggs using. Monodon baculovirus mbv forms polyhedral occlusion bodies obs.
Since 1985, when the first protein il2 was produced in large scale from a recombinant baculovirus. Evidence for the presence of white spot syndrome virus. The present invention relates to the establishment of an immortal shrimp cell line pmo derived from penaeus monodon. World production of shrimp from capture fisheries and aquaculture.